Chin Wee Tan’s PhD Thesis - Appendix Materials

Appendix A1: Computational Models

• Equations and Matlab Codes

Appendix A2: Cellular Volume Microscopy Measurement

• Image processing and analysis procedures
• Matlab routines
• Figures (reproduced from thesis main text for clarity)
• Sample 3D cell volume image stacks (Animations) (Please right-click on the links and click "Save Target As" to save the animation files to your local computer to play)

  Cellular Volume Microscopy Measurement
  
  Typical 3D Tri-stain live cell volume confocal image stacks

  All cells imaged live, tri-stain with Hoechst, Calcein AM and Vybrant DiI.
  

  • HEK293T
  • MDCK
  • SW480
  • SW480APC
  • Caco-2(tri-stain overlay)
  • Caco-2(Hoechst channel)
  • Caco-2(Calcein AM channel)
  • Caco-2(Vybrant DiI channel)

Appendix A3: 3D Quantification: Image processing and analysis

• Procedures
• Matlab routines
• Analysis intermediate results
• Sample 3D image stack (Animations) (Please right-click on the links and click "Save Target As" to save the animation files to your local computer to play)

  3D Quantification: Image processing and analysis

  Typical 3D HEK293T Tri-stain confocal image stack

  • Tri-stain Overlay
  • DAPI channel
  • β-catenin channel
  • N-cadherin channel
• Figures (reproduced from thesis main text for clarity, Chapter 6 and 8)

Appendix A4: HEK293T cell model simulations

• Miscellaneous results for validation and calibration