The Central Resource for Advanced Microscopy is a service core facility to provide microscopy in support of the Institute's research and clinical programs. We have several different forms of light microscopy, many utilizing fluorescence to mark the position of specific proteins of interest. We specialize in time-lapse imaging of live cells and are able to follow these fluorescently tagged proteins within living cells. Confocal microscopy is often used, which is a form of light microscopy, that allows a user to examine inside an intact living cell, without damaging the cell. The technology gives high resolution images, and 3D reconstructions of cells may be made.

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Johnstone, C.N., Tebbutt, N.C., Abud, H.E., White, S.J., Stenvers, K.L., Hall, N.E., Cody, S.H., Whitehead, R.H., Catimel, B., Nice, E.C., Burgess, A.W. and Heath, J.K. Characterization of mouse A33 antigen; a definitive marker for basolateral surfaces of intestinal epithelial cells. American Journal of Physiology 279: G500–G510, (2000).

Cody, S.H., Xiang, S.D., Layton, M.J., Handman, E., Lam, M.H.C., Nice, E.C., and Heath, J.K. A simple method allows DIC imaging in conjunction with confocal microscopy. J. Microscopy. 217: 265-274 (2005).

Cortez, C., Tomaskovic-Crook, E., Johnston A.P.R., Radt B., Cody S.H., Scott, A.M., Nice E.C., Heath, J.K., and Caruso F. Targeting and Uptake of Multilayered Particles to Colorectal Cancer Cells. Advanced Materials. 18: 1998-2003. (2006)

Cannell, M.B. and Cody, S.H. Fluorescent ion measurement. In: Handbook of Biological Confocal Microscopy. 3rd. Edition. (Ed. James Pawley). Chapter 42 (2006).