Proteomics - Platform Technologies - Ludwig Instiute For Cancer Research

The Melbourne Branch (Parkville)

Ludwig Melbourne Branch located in Parkville Melbourne

Research > Platform Technologies >Proteomics


	Angiogenesis
	Signal Transduction
	Epithelial Biochemistry
	Colon Biology
	JPRL
	Cell Biophysics
	LCCI
	Platform Technologies
		Proteomics
		Microscopy
		Biosensors

Platform Technologies - Proteomics

The JPSF has established a world-class protein identification and analysis capability for use by the scientists of WEHI and LICR. The scientists of the JPSF are available to advise scientists on the processes available to enrich particular proteins in biological extracts. JPSF applies powerful electrophoretic, chromatographic, mass spectrometric and bioinformatic analysis techniques to identify the proteins present in complex mixtures. The results generated are reported to scientists in a timely fashion.

The JPSF is located on the 8th Floor of the East-wing of the Royal Melbourne Hospital complex. Potential users of the JPSF are welcome to visit the facilities: simply contact the JPSF Manager, Heather Patsiouras.

For more information visit the Joint Proteomics Services Facility site

Publications of Interest

1. Moritz, R.L., Hall N.E., Connolly L.M., and Simpson, R.J. (2001) Determination of the disulfide structure and N-glycosylation sites of the extracellular domain of the human signal transducer gp130. J Biol Chem. 276: 8244-8253.

2. Varghese, J.N., Moritz, R.L., Lou, M-Z, vanDonkelaar, A., Ji, H., Ivancic, N., Branson, K.M., Hall, N.E., Simpson, R.J. Structure of the extra-cellular domains of the human Interleukin-6 Receptor α Chain. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, pp 15959-15964.

3. Kapp E.A., Schütz, F., Reid, G.E., Eddes J.S.., Moritz, R.L., O’Hair, R.A.J., Speed, T.P. and Simpson R.J Mining a Tandem Mass Spectrometry Database To Determine the Trends and Global Factors Influencing Peptide Fragmentation. (2003) Anal. Chem., 75, pp 6251-6264.

4. Moritz, R.L., Ji, H., Schütz, F., Connolly, L.M. Kapp E.A., Speed, T.P. and Simpson R.J A Proteome fractionation strategy using continuous free-flow electrophoresis coupled off-line to rapid reversed-phase high-performance liquid chromatography. (2004) Anal. Chem., 76, pp 4811-4824.

5. Gilson P.R., Nebl T., Vukcevic D., Moritz R.L., Sargeant T., Speed T.P., Schofield L., Crabb B.S. (2006) Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum. Mol Cell Proteomics. 5 (7): 1286-1299.

Proteomics Laboratory Staff

Facility Managers


Heather Patsiouras Facility Manager	Eugene Kapp Informatics Manager

Staff Directory

group

Heather Patsiouras BSc (Hons)
Position: Facility Manager
Email:

Eugene Kapp MSc
Position: Senior Proteomics Informatics Manager
Email:

Lisa Connolly BSc (Hons)
Position: Proteomics Project Manager
Email:

Guo-Fen Tu PhD
Position: Proteomics technologist
Email:

Giussepe Infusini
Position: Senior Proteomics Technologist
Email:

Grants

Australian Proteomics
Computational Facility

Chief Investigators: Professor Antony Burgess, Dr Robert Moritz, Professor Richard Simpson
Institute: Ludwig Institute for Cancer Research
Funding: $2,000,000 over 5 years

Project description:
In recent years, Australia has invested in the equipment capable of identifying proteins important in health and medicine. This sophisticated equipment requires high capacity computing support.

The Australian Proteomics Computational Facility will establish a single advanced computing cluster accessible to scientists at proteomics centres all over the country. This integrated approach to proteomics computing and the sharing of databases will put Australia at the forefront of the world’s efforts to identify the proteins associated with the early detection of our major diseases