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Receptor Density Analysis by Image Correlation Spectroscopy

Captured By Noga Kozer
Sample A431 Cells
Stain EGFR, AlexaFluor488 (heat map coloured)
Objective UPlanSApo 60x NA 1.35 O (Zoom 7)
Microscope Olympus FV1000 on IX-81 Confocal System
Software Captured with Olympus FluoView (v1.7)
Analysed with ImageJ
Notes
A431 cells were stained for EGFR. Individual cells were captured using photon counting aquisition until they bleached. Cluster density was then analysed using specialist ImageJ plugins for ICS.