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Mouse Ear Montage Scan

Captured By Sophie Parquet-Fifield
Sample Whole Mouse Ear
Stain Green - Anti-Lyve1, AlexaFluor488
  Red - Anti-CD31, AlexaFluor546
Objective PlanFluor 10x
Microscope Nikon Eclipse 90i
Camera Photometrics Coolsnap FX
Software Nikon NIS Elements AR
Notes
Sample was imaged as a montage set of 10x10 fields of view using the moterised stage of the 90i microscope. Images were then analysed with MetaMorph to quantitate staining levels in the whole ear.