Notes

Slide Show

Outline

1	Non-coding RNA or the “art of genome maintenance” Igor Makunin Institute for Molecular Bioscience, University of Queensland
2	Regulation nightmare of eukaryotes
3	Protein-Coding Gene count in genomes
4	Most of the human genome is non-coding
5	How eukaryotes overcome small gene number
6	Why is RNA so attractive?
7	Digital RNA network
8	Parallel output hypothesis
9	Functions of trans-acting RNA signals
10	Epigenetic modification
11	Heterochromatin formation depends on ncRNA
12	Transcription
13	RNA modification: small nucleolar RNAs
14	Alternative splicing
15	MicroRNAs regulate translation and mRNA decay
16	The miRs might participate in Notch pathway
17	Validation of miRs targets in Notch pathway
18	MicroRNA-Directed Cleavage of HOXB8 mRNA
19	Anti-sense transcripts in human HOX clusters
20	Mouse has similar transcript
21	Short RNAs from C. elegans
22	Three major sources of ncRNA in genome: non-coding genes, anti-sense transcripts and introns
23	Mouse has thousands non-coding genes
24	Why has this system gone unnoticed?
25	Non-coding RNA db at the IMB
26	Unusual regions in vertebrate genomes: ultra-conserved elements
27	Ultra-Conserved part of genome
28	We used very stringent criteria
29	The 1045 bp element in POLA gene (human, mouse, rat, dog and chicken)
30	Evolution Slowed for 300+ M Years
31	UC elements are clustered on chromosomes
32	Most UC elements are non-coding but transcribed
33	UC elements associate with regulatory genes
34	Summary
35	Acknowledgments