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The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in more than 80% of human colon cancers and plays a central role in the initiation and progession of colon cancer. The focus of our research is to improve our understanding of the biological function of APC. Our hypothesis is that identification of the proteins that interact with APC at different stages of differentiation and cell migration up the colonic crypt will allow us to understand the role of APC as a tunour suppressor. We aim to identify the regulated formation of APC protein complexes in epithelial cells and to study the dynamics of these interactions to assess their contribution to signalling pathways associated with tumour suppresion.

Actin-cytoskeleton in MDCK Epithelial cells. These cells are used in our studies of APC.

Lab members involved in the research:

• Maree Faux
• Janine Ross
• Melanie Condron
• Ed Nice
• Bruno Catimel
• Tony Burgess

Collaborators:

• Meredith Layton and Richard Simpson, JPSL, Ludwig Institute for Cancer Research
• Alister Sim, University of Newcastle
• John Wade, Howard Florey Institute, Melbourne

Publications:
The use of coiled-coil interactions for the analysis and micropreparative isolation of adenomatous polyposis coli protein complexes.
Catimel B, Faux MC, Nerrie M, Rothacker J, Otvos LJ, Wade JD, Nice EC, Burgess AW.
J Pept Res. 58: 493-503 (2001). [Medline entry]

Structural and biosensor analyses of a synthetic biotinylated peptide probe for the isolation of adenomatous polyposis coli tumor suppressor protein complexes.
Wade JD, Catimel B, Faux MC, Burgess AW, Nice E, Otvos L.
J Pept Res. 58: 204-212 (2001). [Medline entry]