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Epithelial Biochemistry Laboratory Ed Nice LRIC, MIST Associate Member, Principle Research Fellow, Research Manager

Current work: structure-function studies on the EGF/EGF receptor family; pre-clinical studies on AG1478; Analysis of the APC tumour suppressor gene product (purifying APC and APC complexes); role of laminin-10 in colon cancer metastasis; development of novel strategies for protein micropurification/proteomics and biosensor analysis; Purification and characterisation of a putative neutrophil activation factor

Research interests: biosensors; protein-protein interactions; protein kinase regulation

Publication Snapshot:
Crystal structure of a truncated epidermal growth factor receptor extracellular domain bound to transforming growth factor alpha
Garrett TPJ, McKern NM, Lou M, Elleman TC, Adams TE, Lovrecz GO, Zhu H-J, Walker F, Frenkel MJ, Hoyne PA, Jorissen RN, Nice EC, Burgess AW, Ward CW.
Cell. 110: 763-773 (2002). [Medline entry]

Rapid microscale enzymic semisynthesis of epidermal growth factor (EGF) analogues.
Nice EC, Domagala T, Fabri L, Nerriea M, Walker F, Jorissen RN, Burgess AW, Cui DF, Zhang YS.
Growth Factors. 20: 71-80 (2002). [Medline entry]

The use of coiled-coil interactions for the analysis and micropreparative isolation of adenomatous polyposis coli protein complexes.
Catimel B, Faux MC, Nerrie M, Rothacker J, Otvos LJ, Wade JD, Nice EC, Burgess AW.
J Pept Res. 58: 493-503 (2001). [Medline entry]

Novel monoclonal antibody specific for the de2-7 epidermal growth factor receptor (EGFR) that also recognizes the EGFR expressed in cells containing amplification of the EGFR gene.
Johns TG, Stockert E, Ritter G, Jungbluth AA, Huang HJ, Cavenee WK, Smyth FE, Hall CM, Watson N, Nice EC, Gullick WJ, Old LJ, Burgess AW, Scott AM.
Int J Cancer. 98: 398-408 (2002). [Medline entry]

Biosensors and Proteomics.
Catimel B, Rothacker J, Nice EC.
Todays Life Science. 14(1): 24-30 (2002).

Laminin-10 mediates basal and EGF-stimulated motility of human colon carcinoma cells via alpha(3)beta(1) and alpha(6)beta(4) integrins.
Pouliot N, Nice EC, Burgess AW.
Exp Cell Res. 266: 1-10 (2001). [Medline entry]

Biophysical characterization of interactions involving importin-alpha during nuclear import.
Catimel B, Teh T, Fontes MR, Jennings IG, Jans DA, Howlett GJ, Nice EC, Kobe B.
J Biol Chem. 276: 34189-34198 (2001). [Medline entry]

Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3. Mäkinen T, Veikkola T, Mustjoki S, Karpanen T, Catimel B, Nice EC, Wise L, Mercer A, Kowalski H, Kerjaschki D, Stacker SA, Achen MG, Alitalo K. EMBO J. 2001 Sep 3;20(17):4762-73. [Medline entry]

The use of biosensors for microaffinity purification: an integrated approach to proteomics.
Catimel B, Rothacker J, Nice E.
J Biochem Biophys Methods. 49: 289-312 (2001). [Medline entry]

Structural and biosensor analyses of a synthetic biotinylated peptide probe for the isolation of adenomatous polyposis coli tumor suppressor protein complexes.
Wade JD, Catimel B, Faux MC, Burgess AW, Nice E, Otvos L.
J Pept Res. 58:204-212 (2001). [Medline entry]

Identification of a determinant of epidermal growth factor receptor ligand-binding specificity using a truncated, high-affinity form of the ectodomain.
Elleman TC, Domagala T, McKern NM, Nerrie M, Lönnqvist B, Adams TE, Lewis J, Lovrecz GO, Hoyne PA, Richards KM, Howlett GJ, Rothacker J, Jorissen RN, Lou M, Garrett TPJ, Burgess AW, Nice EC*, Ward CW*.
Biochemistry 40:8930-8939 (2001). [Medline entry]
* Both are corresponding authors

Specific localization, gamma camera imaging, and intracellular trafficking of radiolabelled chimeric anti-G(D3) ganglioside monoclonal antibody KM871 in SK-MEL-28 melanoma xenografts.
Lee FT, Rigopoulos A, Hall C, Clarke K, Cody SH, Smyth FE, Liu Z, Brechbiel MW, Hanai N, Nice EC, Catimel B, Burgess AW, Welt S, Ritter G, Old LJ, Scott AM.
Cancer Res. 61: 4474-4482 (2001). [Medline entry]

Design of inhibitors of Ras-Raf interaction using a computational combinatorial algorithm.
Zeng J, Nheu T, Zorzet A, Catimel B, Nice E, Maruta H, Burgess AW, Treutlein HR.
Protein Eng. 14: 39-45 (2001). [Medline entry]

The specificity of receptor binding by vascular endothelial growth factor-D is different in mouse and man.
Baldwin ME, Catimel B, Nice EC, Roufail S, Hall NE, Stenvers KL, Karkkainen MJ, Alitalo K, Stacker SA, Achen MG.
J Biol Chem. 276: 19166-19171 (2001). [Medline entry]

Targeting properties of an anti-CD16/anti-CD30 bispecific antibody in an in vivo system.
Renner C, Stehle I, Lee FT, Hall C, Catimel B, Nice EC, Mountain A, Rigopoulos A, Brechbiel MW, Pfreundschuh M, Scott AM.
Cancer Immunol Immunother. 50: 102-108 (2001). [Medline entry]

Synthesis, conformational studies and biological activity of N(alpha)-mono-biotinylated rat relaxin.
Mathieu MN, Wade JD, Catimel B, Bond CP, Nice EC, Summers RJ, Otvos L Jr, Tregear GW.
J Pept Res. 57: 374-382 (2001). [Medline entry]

High-performance liquid chromatographic analysis of the tyrphostin AG1478, a specific inhibitor of the epidermal growth factor receptor tyrosine kinase, in mouse plasma.
Ellis AG, Nice EC, Weinstock J, Levitzki A, Burgess AW, Webster LK.
J Chromatogr B Biomed Sci Appl. 754: 193-9 (2001). [Medline entry]

c-Raf-1 RBD associates with a subset of active v-H-Ras.
Fridman M, Walker F, Catimel B, Domagala T, Nice E, Burgess A.
Biochemistry. 39: 15603-15611 (2000). [Medline entry]

Construction, production, and characterization of humanized anti-Lewis Y monoclonal antibody 3S193 for targeted immunotherapy of solid tumors.
Scott AM, Geleick D, Rubira M, Clarke K, Nice EC, Smyth FE, Stockert E, Richards EC, Carr FJ, Harris WJ, Armour KL, Rood J, Kypridis A, Kronina V, Murphy R, Lee FT, Liu Z, Kitamura K, Ritter G, Laughton K, Hoffman E, Burgess AW, Old LJ.
Cancer Res. 60: 3254-3261 (2000). [Medline entry]

Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells.
Johnstone CN, Tebbutt NC, Abud HE, White SJ, Stenvers KL, Hall NE, Cody SH, Whitehead RH, Catimel B, Nice EC, Burgess AW, Heath JK.
Am J Physiol Gastrointest Liver Physiol. 279: G500-G510 (2000). [Medline entry]

Monoclonal antibodies to vascular endothelial growth factor-D block its interactions with both VEGF receptor-2 and VEGF receptor-3.
Achen MG, Roufail S, Domagala T, Catimel B, Nice EC, Geleick DM, Murphy R, Scott AM, Caesar C, Makinen T, Alitalo K, Stacker SA.
Eur J Biochem. 267: 2505-2515 (2000). [Medline entry]

Micropreparative ligand fishing with a cuvette-based optical mirror resonance biosensor.
Catimel B, Weinstock J, Nerrie M, Domagala T, Nice EC.
J Chromatogr A 869: 261-273 (2000). [Medline entry]

Recent applications of instrumental biosensors for protein and peptide structure-function studies
Catimel B, Domagala T, Nerrie M, Weinstock J, White S, Abud H, Heath J, Nice EC.
Protein and Peptide Letters. 6: 319-340 (1999).

A mutant form of vascular endothelial growth factor (VEGF) that lacks VEGF receptor-2
activation retains the ability to induce vascular permeability.
Stacker SA, Vitali A, Caesar C, Domagala T, Groenen LC, Nice E, Achen MG, Wilks AF. J Biol Chem. 274: 34884-34892 (1999). [Medline entry]

Biosynthesis of vascular endothelial growth factor-D involves proteolytic processing which generates non-covalent homodimers.
Stacker SA, Stenvers K, Caesar C, Vitali A, Domagala T, Nice E, Roufail S, Simpson RJ, Moritz R, Karpanen T, Alitalo K, Achen MG.
J Biol Chem. 274: 32127-32136 (1999). [Medline entry]

Stoichiometry, kinetic and binding analysis of the interaction between epidermal growth factor (EGF) and the extracellular domain of the EGF receptor.
Domagala T, Konstantopoulos N, Smyth F, Jorissen RN, Fabri L, Geleick D, Lax I, Schlessinger J, Sawyer W, Howlett GJ, Burgess AW, Nice EC.
Growth Factors. 18: 11-29 (2000). [Medline entry]

Analysis of the binding of the Fab fragment of monoclonal antibody NC10 to influenza virus N9 neuraminidase from tern and whale using the BIAcore biosensor: effect of immobilization level and flow rate on kinetic analysis.
Kortt AA, Nice E, Gruen LC.
Anal Biochem. 273: 133-141 (1999). [Medline entry]

Applications of optical biosensors to structure-function studies in the EGF/EGF receptor system.
Nice EC, Domagala T, Smyth FE, Konstantopoulos N, Nerrie M, Geleick D, Catimel B, Burgess AW.
Peptides: biology and chemistry. Proceedings of the 1998 Chinese Peptide Symposium, July 14-17, 1998, Lanzhou, China. 238-243 (2000) London. Publisher: Kluwer Academic. Editors: X-Yu Hu, R Wang and JP Tam.

Point mutants of c-raf-1 RBD with elevated binding to v-Ha-Ras.
Fridman M, Maruta H, Gonez J, Walker F, Treutlein H, Zeng J, Burgess A.
J Biol Chem. 275:30363-30371 (2000). [Medline entry]

Instrumental biosensors: new perspectives for the analysis of biomolecular interactions.
Nice EC, Catimel B.
Bioessays. 21: 339-352 (1999). [Medline entry]