Epithelial lab front page

Lab head

Current Research

Laboratory staff

Selected Publications

New Stuff

We have had a long term focus on the development of novel methods for the micropurification of proteins and peptides for structure-function and proteomics studies, and in the development of biosensor methodologies (using the BIAcore, IAsys and Cytosensor methodologies) to measure protein-protein interactions and for ligand fishing. These methods have been applied to the study of a number of receptor ligand systems implicated in cancer (eg EGF/EGFR family, the haemopoietic CSFs eg G-CSF, GM-CSF) and their down stream signalling pathways.

Biosensor curves for the binding of soluble EGF receptor ectodomain to immobilized rhEGF at different concentrations. From this data, the rates of association and dissociation can be determined.

Lab members involved in the research:

• Ed Nice
• Bruno Catimel
• Julie Rothacker
• Maureen Nerrie

Publications:
Biosensors and Proteomics.
Catimel B, Rothacker J, Nice EC.
Todays Life Science. 14(1): 24-30 (2002).

The use of biosensors for microaffinity purification: an integrated approach to proteomics.
Catimel B, Rothacker J, Nice E.
J Biochem Biophys Methods. 49: 289-312 (2001). [Medline entry]

Micropreparative ligand fishing with a cuvette-based optical mirror resonance biosensor.
Catimel B, Weinstock J, Nerrie M, Domagala T, Nice EC.
J Chromatogr A. Feb 869:261-273 (2000).
[Medline entry]

Instrumental biosensors: new perspectives for the analysis of biomolecular interactions.
Nice EC, Catimel B.
Bioessays. 21:339-352 (1999). [Medline entry]

Kinetic analysis of the interaction between the monoclonal antibody A33 and its colonic epithelial antigen by the use of an optical biosensor. A comparison of immobilisation strategies.
Catimel B, Nerrie M, Lee FT, Scott AM, Ritter G, Welt S, Old LJ, Burgess AW, Nice EC.
J Chromatogr A. 776:15-30 (1997). [Medline entry]

Gruen LC, McKimm-Breschkin JL, Caldwell JB, Nice EC.
Affinity ranking of influenza neuraminidase mutants with monoclonal antibodies using an optical biosensor. Comparison with ELISA and slot blot assays.
J Immunol Methods. 168:91-100 (1994). [Medline entry]

Micropreparative ion exchange high performance liquid chromatography: applications to microsequence analysis.
Nice EC, Fabri L, Hammacher A, Andersson K, Hellman U.
Biomed Chromatogr. 7:104-111 (1993). [Medline entry]

Micropreparative LC of proteins.
Nice EC.
Nature. 348: 462-463 (1990).

Simpson RJ, Moritz RL, Begg GS, Rubira MR, Nice EC.
Micropreparative procedures for high sensitivity sequencing of peptides and proteins.
Anal Biochem. 177:221-236 (1989). [Medline entry]

Nice EC, Simpson RJ.
Micropreparative high-performance liquid chromatography of proteins and peptides.
J Pharm Biomed Anal. 7:1039-1053 (1989). [Medline entry]